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In combination with TAs, the CAR 元42E protein showed 50–75% conversion of 6-ACA to HMD. To increase the cascade activity for the transformation of 6-ACA to HMD, we determined the crystal structure of the CAR substrate-binding domain in complex with AMP and succinate and engineered three mutant CARs with enhanced activity against 6-ACA. Using purified wild type CARs and TAs supplemented with cofactor regenerating systems for ATP, NADPH, and amine donor, we established a one-pot enzyme cascade catalyzing up to 95% conversion of AA to 6-ACA. Here we report the biocatalytic conversion/transformation of AA to 6-ACA and HMD by carboxylic acid reductases (CARs) and transaminases (TAs), which involves two rounds (cascades) of reduction/amination reactions (AA → 6-ACA → HMD). In recent years, the biosynthesis of adipic acid from renewable feedstocks has been demonstrated using both bacterial and yeast cells. Adipic acid (AA), 6-aminocaproic acid (6-ACA) and 1,6-hexamethylenediamine (HMD) are key precursors for nylon synthesis, which are currently produced primarily from petroleum-based feedstocks. Production of platform chemicals from renewable feedstocks is becoming increasingly important due to concerns on environmental contamination, climate change, and depletion of fossil fuels. I hope somebody know what was it called or can help me to find it. Website: CodeShout Network - TWars v2.0 - Free Massive Multiplayer Online Game where you need a browser and an internet connection in order to play!ĭescription: CodeShout: Trinity Wars - The War Has Just Begun - Massive Multiplayer Online Space Role Playing Game - you only need a browser and an internet connection in order to play! Year 2070 - The Universe Is Your- O-game Type ServerĪ long time ago i have seen something, that looked like a space strategy game or anything like that. 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